Yiwei Xiaoyu granules mitigate spasmolytic polypeptide-expressing metaplasia-associated gastric mucosal damage by regulating WFDC2 and the miRNA-7/circRNA network

Figure 6 WFDC2 expression in gastric mucosa at days 15 and 30. A: Relative WFDC2 mRNA expression levels at days 15 and 30 in different experimental groups. At day 15: ^aP < 0.01 vs G2, ^bP < 0.0001 vs G3, ^cP < 0.01 vs G5, ^dP < 0.0001 vs G4, ^eP < 0.0001 vs G5. At day 30: ^fP < 0.001 vs G2, ^gP < 0.0001 vs G5, ^hP < 0.0001 vs G5; B: Representative fluorescence in situ hybridization (FISH) images showing WFDC2 expression in gastric mucosa across experimental groups. Scale bar = 100 µm; C: Quantification of WFDC2-positive area (%Area) across experimental groups. ^mP < 0.001, ⁿP < 0.001 vs G5. Data are presented as mean ± SD (n = 5 per group). Statistical analysis was performed using two-way analysis of variance followed by Tukey’s multiple-comparisons test.