Hsa_circ_0003848 promotes gastric cancer via miR-144-3p/mesenchymal-epithelial transition factor and serves as a plasma biomarker in Helicobacter pylori infection

Figure 3 Effects of hsa_circ_0003848 on gastric cancer cell function. A: Real-time quantitative PCR shows higher expression of hsa_circ_0003848 in gastric cancer cells compared to normal GES-1 cells; B: Knockdown efficiency of hsa_circ_0003848 confirmed in GES-1, AGS, and HGC-27 cells using siRNAs; C: Cell counting kit-8 assay shows reduced proliferation in AGS and HGC-27 cells after knockdown; D: Colony formation assay indicates decreased clonogenic ability in AGS and HGC-27 after knockdown; E: Transwell assay shows reduced migration of gastric cancer cells after knockdown; F: Flow cytometry reveals increased apoptosis in AGS and HGC-27 cells after knockdown; G: Cell cycle analysis shows G1 phase arrest in AGS and HGC-27 cells after knockdown. n = 3, ^bP < 0.01, ^cP < 0.001. NC: Negative control.