FNDC5 enhances quercetin-induced anoikis in pancreatic adenocarcinoma cells via focal adhesion kinase-dependent mechanisms

Figure 4 FNDC5 reduced focal adhesion kinase signaling pathway and promotes quercetin-induced anoikis in pancreatic ductal adenocarcinoma cells. A: Western blot detected the anoikis apoptosis related proteins in Capan-1-F5, MiaPaca-2-F5, and their control cells treated with 100 nM quercetin or dimethyl sulfoxide; B and C: Real-time cellular analysis assay showed that high FNDC5 led to a decrease in cell proliferation (Capan-1-F5, MiaPaca-2-F5) after quercetin treatment. ^bP < 0.01. ^cP < 0.001. NS: Not significant; QUN: Quercetin; DMSO: Dimethyl sulfoxide; FAK: Focal adhesion kinase; P-FAK: Phospho-focal adhesion kinase; AKT: Protein kinase B; P-AKT: Phospho-protein kinase B; PI3K: Phosphatidylinositol 3-kinase; P-PI3K: Phospho-phosphatidylinositol 3-kinase.