FNDC5 enhances quercetin-induced anoikis in pancreatic adenocarcinoma cells via focal adhesion kinase-dependent mechanisms

Figure 1 FNDC5 expression is correlate with better overall survival of pancreatic adenocarcinoma patients. A: Based on the Cancer Genome Atlas database, the box plot showed that FNDC5 was highly expressed in pancreatic ductal adenocarcinoma (PAAD) tissues; B: Violin plot displaying FNDC5 expression levels stratified by clinical stages of PAAD (stage I-IV); C: Kaplan-Meier curve showed that FNDC5 expression was associated with overall survival in PAAD patients; D: Kaplan-Meier curve showed that FNDC5 expression was associated with progression-free survival in PAAD patients; E: Heat map showed that FNDC5 was a protective factor for PAAD by Cox regression analysis; F: Real-time quantitative polymerase chain reaction analysis showing gene expression of FNDC5 in PAAD cell lines (Capan-1, PANC-1, SU86.86, and MiaPaCa-2); G: Western blot analysis showing protein levels of irisin in PAAD cell lines. ^bP < 0.01. ^cP < 0.001. TPM: Transcripts per million; PAAD: Pancreatic ductal adenocarcinoma; HR: Hazard ratio; OS: Overall survival; KM: Kaplan-Meier; mRNA: Messenger RNA; ACC: Adrenocortical carcinoma; BLCA: Bladder urothelial carcinoma; BRCA: Breast invasive carcinoma; CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma; CHOL: Cholangiocarcinoma; COAD: Colon adenocarcinoma; DLBC: Lymphoid neoplasm diffuse large B-cell lymphoma; ESCA: Esophageal carcinoma; GBM: Glioblastoma multiforme; HNSC: Head and neck squamous cell carcinoma; KICH: Kidney chromophobe; KIRC: Kidney renal clear cell carcinoma; KIRP: Kidney renal papillary cell carcinoma; LAML: Acute myeloid leukemia; LGG: Brain lower grade glioma; LIHC: Liver hepatocellular carcinoma; LUAD: Lung adenocarcinoma; LUSC: Lung squamous cell carcinoma; MESO: Mesothelioma; OV: Ovarian serous cystadenocarcinoma; PCPG: Pheochromocytoma and paraganglioma; PRAD: Prostate adenocarcinoma; READ: Rectum adenocarcinoma; SARC: Sarcoma; SKCM: Skin cutaneous melanoma; STAD: Stomach adenocarcinoma; TGCT: Testicular germ cell tumors; THCA: Thyroid carcinoma; THYM: Thymoma; UCEC: Uterine corpus endometrial carcinoma; UCS: Uterine carcinosarcoma; UVM: Uveal melanoma.