Metastasis-associated colon cancer 1 drives pancreatic cancer via p53/Notch signaling

Figure 8 Metastasis-associated colon cancer 1 activated Notch and p53 signaling pathway. A and B: Hallmarks of gene set enrichment analyses revealed that the expression level of Metastasis-associated colon cancer 1 significantly correlates with Notch and p53 signaling pathway; C: The luciferase assay showed the effect of Metastasis-associated colon cancer 1 on luciferase activity of Notch1 and p53 promoter in ASPC-1; D: Up: Western blot result about the expression of Notch pathway-related protein, including Notch1, Hey1 and Hes1 in downregulated ASPC-1, and western blot result about the expression of p53 pathway-related protein downregulated in ASPC-1. Down: The gray density about the expression of Notch pathway-related protein, including Notch1, Hey1 and Hes1 in downregulated ASPC-1 (n = 3), and the gray density about the expression of p53 pathway-related protein (n = 3). ^aP < 0.05, ^bP < 0.01. FDR: False discovery rate; KEGG: Kyoto Encyclopedia of Genes and Genomes; MACC1: Metastasis-associated colon cancer 1; NES: Normalized enrichment score; NS: Not significant.