Dysregulation of the HIF-1α/BNIP3 axis links defective mitophagy to glycolytic reprogramming in a rat model of gastric precancerogenesis

Figure 7 Dynamic changes in the protein expression of key markers in the hypoxia-inducible factor 1-alpha/BCL2/adenovirus E1B 19kDa interacting protein 3 signaling pathway in the rat gastric mucosa. The relative protein expression of hypoxia-Inducible Factor 1-alpha, BCL2/adenovirus E1B 19 kDa interacting protein 3, Beclin1, microtubule-associated protein 1 light chain 3 II, sequestosome 1, cytochrome C oxidase subunit 4, hexokinase 2, and lactate dehydrogenase A was determined by western blot analysis. Data are presented as the mean ± SD from three independent animals per group. ^aP < 0.05, ^bP < 0.01 vs the normal group. BNIP3: BCL2/adenovirus E1B 19 kDa interacting protein 3; COX4: Cytochrome C oxidase subunit 4; HIF-1α: Hypoxia-inducible factor 1-alpha; HK2: Hexokinase 2; LC3: Light chain 3; LDHA: Lactate dehydrogenase A; P62/SQSTM1: Sequestosome 1.