MicroRNA-206 suppresses hypoxia-inducible factor-1α/PFKFB3-mediated glycolysis to inhibit recurrence and metastasis of hepatocellular carcinoma after incomplete radiofrequency ablation

Figure 5 Angiogenesis, glycolytic signaling, and protein expression in rabbit VX2 liver tumors after complete or incomplete radiofrequency ablation. A: Immunofluorescence staining of α-SMA in sham, complete radiofrequency ablation (RFA), and incomplete RFA groups at 0 week, 1 week, and 3 weeks (n = 6 animals per group per timepoint); B: Quantification of α-SMA average optical density (AOD) (n = 6); C: Western blot analysis of hypoxia-inducible factor-1α (HIF-1α), 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3, and GAPDH across treatment groups and timepoints (n = 6); D: Immunohistochemistry (IHC) of HIF-1α expression at each timepoint (n = 6); E: Quantification of HIF-1α IHC AOD (n = 6). Data are shown as mean ± SD; one-way ANOVA was used for comparisons. ^aP < 0.05. RFA: Radiofrequency ablation; HIF-1α: Hypoxia-inducible factor-1α; PFKFB3: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; AOD: Average optical density.