MicroRNA-206 suppresses hypoxia-inducible factor-1α/PFKFB3-mediated glycolysis to inhibit recurrence and metastasis of hepatocellular carcinoma after incomplete radiofrequency ablation

Figure 4 Effects of microRNA-206 overexpression and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 knockdown on endothelial function and glycolysis under thermal stimulation. A: Tube-formation assay under 37 °C and 41 °C in control, mimic NC, microRNA-206 (miR-206) mimics, shRNA NC, and sh-6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) groups (n = 3); B and C: TUNEL apoptosis staining and quantification under the same conditions (n = 3); D: Transwell migration assay results comparing miR-206 and sh-PFKFB3 effects (n = 3); E: Western blot analysis of hypoxia-inducible factor-1α, PFKFB3, and GAPDH under all conditions (n = 3). Data represent mean ± SD; one-way ANOVA was used. ^aP < 0.05. Td-EC: Tumor-derived endothelial cells; HIF-1α: Hypoxia-inducible factor-1α; PFKFB3: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3.