Morphological and functional responses of three-dimensional-cultured colorectal cancer spheres to anticancer drugs

Figure 8 Effects of three anticancer drugs on the growth of three-dimensional-cultured colorectal cancer spheres. DLD-1, CACO-2, HCT-15, SW480, and COLO-320 spheres were imaged using a three-dimensional (3D) imaging system on days 0, 1, 3, 5, and 7 after treatment with three anticancer drugs (5-fluorouracil, irinotecan, and oxaliplatin). Sphere areas were quantified from the 3D images using an integrated artificial intelligence-based deep learning algorithm. Solid lines indicate anticancer drug-treated groups, and dashed lines indicate the corresponding control groups. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; ^dP < 0.0001. 5-FU: 5-fluorouracil.