Morphological and functional responses of three-dimensional-cultured colorectal cancer spheres to anticancer drugs

doi:10.4251/wjgo.v18.i4.116097

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World Journal of Gastrointestinal Oncology

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1948-5204

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.

World J Gastrointest Oncol. Apr 15, 2026; 18(4): 116097
Published online Apr 15, 2026. doi: 10.4251/wjgo.v18.i4.116097

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Figure 1 Schema of the experimental method. Colorectal adenocarcinoma cells were cultured in low-attachment 96-well plates for 3 days to form spheres. After sphere formation, anticancer drugs were added to each well at a concentration of 30 μM. The day of anticancer drug administration was defined as day 0, and the areas of the spheres were measured on days 0, 1, 3, 5, and 7 using an imaging system. 5-FU: 5-fluorouracil; WST-8: Water-soluble tetrazolium 8; qRT-PCR: Quantitative reverse transcriptase PCR; CSC: Cancer stem cell; EMT: Epithelial-mesenchymal transition.

Citation: Yoshimura Y, Shinji S, Ogawa Y, Yamada T, Matsuda A, Uehara K, Yokoyama Y, Takahashi G, Iwai T, Miyasaka T, Kanaka S, Matsui T, Hayashi K, Fujiwara M, Shichi Y, Arai T, Ishiwata T, Yoshida H. Morphological and functional responses of three-dimensional-cultured colorectal cancer spheres to anticancer drugs. World J Gastrointest Oncol 2026; 18(4): 116097
URL: https://www.wjgnet.com/1948-5204/full/v18/i4/116097.htm
DOI: https://dx.doi.org/10.4251/wjgo.v18.i4.116097

Yoshimura Y, Shinji S, Ogawa Y, Yamada T, Matsuda A, Uehara K, Yokoyama Y, Takahashi G, Iwai T, Miyasaka T, Kanaka S, Matsui T, Hayashi K, Fujiwara M, Shichi Y, Arai T, Ishiwata T, Yoshida H. Morphological and functional responses of three-dimensional-cultured colorectal cancer spheres to anticancer drugs. World J Gastrointest Oncol 2026; 18(4): 116097 [DOI: 10.4251/wjgo.v18.i4.116097]

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