HMGCR loss is synthetic lethal with PIK3CD inhibition in colorectal cancer cells

Figure 2 Atorvastatin combined with gefitinib had an enhanced inhibitory effect on colorectal cancer cell metastasis and promotes autophagy. A and B: Transwell assay showed that compared with atorvastatin or gefitinib alone, atorvastatin combined with gefitinib increased the inhibition of SW480 cells and HCT116 cell metastasis; C and D: Western blotting results showed that after atorvastatin and gefitinib treatment, the expression of E-cadherin increased, and the expression of vimentin decreased, compared with atorvastatin or gefitinib alone. The combination of atorvastatin and gefitinib had a stronger effect on protein expression; E: Compared with atorvastatin or gefitinib alone, the combination of atorvastatin and gefitinib showed more autophagy spots; F and G: Western blotting showed that compared with atorvastatin or gefitinib alone, the combination of atorvastatin and gefitinib increased the expression of autophagy-related genes LC3-II/I and Beclin-1, and decreased the expression of P62. Data are presented as the mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001; NS: Not significant.