Oncogenic CD44 is essential for pancreatic cancer tumorigenesis: A novel targeted therapeutic strategy

Figure 3 CD44 knockout inhibits pancreatic cancer cell migration and invasion. Pancreatic cancer Patu-8988 and Panc-1 cells were cultured in six-well plates for a wound-healing assay. A-D: The cell monolayer was photographed at 0 hour and 24 hours of the assay, respectively, and the number of migrated cells was counted and statistically analyzed. In the cell invasion assay, Patu-8988 and Panc-1 cells were cultured in Transwell chambers pre-coated with 100 mL/L Matrigel and incubated for 24 hours; E-H: The invaded cells were stained by Giemsa staining and photographed under an orthostatic microscope (200 ×), and the number of invaded cells was counted and statistically analyzed. Data are shown as the mean ± SD of three independent replicates. ^aP < 0.05, ^bP < 0.01 vs CD44-NT in an unpaired t test.