Yiqi Wenyang Jiedu prescription promotes disulfidptosis of gastric cancer by reversing senescent cancer-associated fibroblasts

Figure 2 Establishment of the senescent cancer-associated fibroblasts model and intervention effect of Yiqi Wenyang Jiedu prescription. A: Proportion of β-galactosidase staining-positive cells in the cancer-associated fibroblasts (CAFs) model and senescent CAFs (senCAFs) model under the same scale bar; B: Reversal effect on senCAFs by Yiqi Wenyang Jiedu prescription (YWJP)-containing serum at different concentrations vs blank serum (BS); C: Secretion levels of interleukin-6 (IL-6), interleukin-8 (IL-8), hyaluronic acid (HA), and HAase in senCAFs treated with YWJP at different concentrations vs BS, detected by ELISA; D: MRNA expression levels of IL-6, IL-8, HA, and HAase in senCAFs treated with YWJP at different concentrations vs BS, detected by quantitative PCR. ^aP < 0.05, ^bP < 0.01, NS: Not significant. Error bars represent the standard error of the data. CAF: Cancer-associated fibroblasts; YWJP: Yiqi Wenyang Jiedu prescription; BS: Blank serum; senCAFs: Senescent cancer-associated fibroblasts; IL-6: Interleukin-6; IL-8: Interleukin-8; HA: Hyaluronic acid.