Goosecoid drives colorectal cancer progression by inducing epithelial-mesenchymal transition

Figure 1 Prognostic significance and differential expression of goosecoid in colorectal cancer. A: UALCAN database analysis showed that goosecoid (GSC) protein was significantly upregulated in colorectal cancer (CRC); B: Immunohistochemical staining revealing GSC expression in CRC specimens compared to matched non-cancerous tissues. Scale bar: 50 μm (20 ×); scale bar: 20 μm (40 ×); C: Immunofluorescence revealing GSC expression patterns and subcellular distribution in CRC tissues. Scale bar: 20 μm (40 ×); D: Immunoblot analysis of GSC protein levels in three paired CRC/normal tissue samples; E: GSC expression profiles across CRC cell lines (HCT29, HCT8, SW480, HCT116, and DLD-1) and normal colonic NCM460 cells; F: Kaplan-Meier curves demonstrating correlation between elevated GSC expression and reduced 10-year overall and recurrence-free survival. All data are the mean ± SD from three independent experiments. ^aP < 0.05. COAD: Colon adenocarcinoma; CRC: Colorectal cancer; GSC: Goosecoid; READ: Rectal adenocarcinoma; TCGA: The Cancer Genome Atlas.