Resveratrol suppresses ulcerative colitis and colitis-associated colorectal cancer via modulating the miR-31-5p/SATB2 axis

Figure 2 MiR-31-5p modulates migration, proliferation, apoptosis, and related protein expression in HCT116 colorectal cancer cells. A: Quantitative reverse transcription polymerase chain reaction analysis of miR-31-5p expression in HCT116 cells transfected with miR-31-5p mimic or inhibitor (n = 3); B: Wound healing assay showing the effect of miR-31-5p on HCT116 cell migration (scale bar = 200 μm) (n = 3); C: Colony formation assay showing the effect of miR-31-5p on HCT116 cell proliferation (n = 4); D: Flow cytometric analysis of the effect of miR-31-5p on HCT116 cell apoptosis (n = 3); E: Western blotting analysis of the effect of miR-31-5p on apoptosis-related protein expression in HCT116 cells (n = 3). ^aP < 0.05 vs control, ^bP < 0.01 vs control, ^eP < 0.01 vs miR-31-5p mimic (30 nM), ^hP < 0.01 vs miR-31-5p inhibitor (50 nM).