Resveratrol suppresses ulcerative colitis and colitis-associated colorectal cancer via modulating the miR-31-5p/SATB2 axis

Figure 1 MiR-31-5p expression levels are elevated in human colorectal cancer, human ulcerative colitis, mouse colitis-associated colorectal cancer, and HCT116 colorectal cancer cells. A: The Cancer Genome Atlas-derived box plot of miR-31-5p expression in colorectal cancer tissues vs normal controls. ^cP < 0.001 vs normal controls; B: Heatmap of differentially expressed microRNAs in a pilot human ulcerative colitis (UC) vs adjacent normal controls; C: Volcano plot of differentially expressed microRNAs in a pilot human UC vs adjacent normal controls; D: MiR-31-5p expression in paired inflamed and adjacent normal controls colonic tissues from pilot UC patients. ^aP < 0.05 vs adjacent normal controls (n = 4 pairs); E: Quantitative reverse transcription polymerase chain reaction quantification of colonic miR-31 in azoxymethane/dextran sulfate sodium-treated C57BL/6 mice. ^bP < 0.01 vs control (n = 7); F: Quantitative reverse transcription polymerase chain reaction quantification of miR-31-5p expression in NCM-460 cells and HCT116 cells. ^bP < 0.01 vs NCM-460 (n = 7). N: Normal controls; UC: Ulcerative colitis; AOM: Azoxymethane; DSS: Dextran sulfate sodium.