Microbial succession driven by Alistipes depletion shapes the mesenchymal and immune-excluded consensus molecular subtype 4 colorectal cancer landscape

Figure 4 Immune infiltration patterns and functional immunological features across Consensus Molecular Subtypes classifications. A: Z-score normalized enrichment scores of 24 immune cell types estimated by single-sample gene set enrichment analysis; B: Heatmap comparing infiltration levels of representative immune cells among Consensus Molecular Subtypes (CMS1)-CMS4; C: Boxplots showing subtype-specific differences for five significantly different immune cell types; D: Correlations between top five subtype-specific marker genes and immune infiltration within each CMS; E: Violin plots of programmed death-ligand 1 response probability, cytolytic activity score, immune exclusion score, and immunophenoscore across CMS classifications. CMS: Consensus Molecular Subtypes; NK: Natural killer; ssGSEA: Single-sample gene set enrichment analysis; PD-1: Programmed cell death protein 1; CYT: Cytolytic activity score; IPS: Immunophenoscore.