Angelica sinensis polysaccharide attenuates constipation-associated colorectal carcinogenesis by inhibiting the NLRP3/Caspase-1/IL-1β inflammasome axis

Figure 6 Combined pathway modulation confirms the NOD-like receptor protein 3/caspase-1/interleukin-1β axis as the target of Angelica sinensis polysaccharide. A: Representative immunofluorescence images showing CK20 (green), cleaved caspase-1 p20 (red), DAPI (blue), and merged images in control, deoxycholic acid, deoxycholic acid + Angelica sinensis polysaccharide (ASP), ASP + nigericin, ASP + nigericin + adenosine 5′-triphosphate disodium salt, and ASP + nigericin + VX-765 groups; B: Quantification of cleaved caspase-1 relative fluorescence intensity. ^aP < 0.05. DCA: Deoxycholic acid; ASP: Angelica sinensis polysaccharide; ATP: Adenosine 5′-triphosphate disodium salt; CA19-9: Carbohydrate antigen 19-9.