Angelica sinensis polysaccharide attenuates constipation-associated colorectal carcinogenesis by inhibiting the NLRP3/Caspase-1/IL-1β inflammasome axis

Figure 5 Caspase-1 inhibitor VX-765 blocks interleukin-1β expression while extracellular adenosine 5′-triphosphate disodium salt enhances it. A: Representative immunofluorescence images showing CK20 (green), interleukin-1β (red), DAPI (blue), and merged images in control, culture in vehicle adding equimolar sodium chloride to control for osmolality, deoxycholic acid, Angelica sinensis polysaccharide (ASP), ASP + adenosine 5′-triphosphate disodium salt, and ASP+VX-765 groups; B: Quantification of interleukin-1β relative fluorescence intensity. ^aP < 0.05. OC: Culture in vehicle adding equimolar sodium chloride to control for osmolality; DCA: Deoxycholic acid; ASP: Angelica sinensis polysaccharide; ATP: Adenosine 5′-triphosphate disodium salt; CA19-9: Carbohydrate antigen 19-9.