Angelica sinensis polysaccharide attenuates constipation-associated colorectal carcinogenesis by inhibiting the NLRP3/Caspase-1/IL-1β inflammasome axis

Figure 4 NOD-like receptor protein 3 inhibitor MCC950 attenuates nigericin-induced caspase-1 activation in Angelica sinensis polysaccharide-treated cells. A: Representative immunofluorescence images showing CK20 (green), cleaved caspase-1 p20 (red), DAPI (blue), and merged images in control, culture in vehicle adding equimolar sodium chloride to control for osmolality, deoxycholic acid, Angelica sinensis polysaccharide (ASP), ASP + nigericin, and ASP + MCC950 groups; B: Quantification of cleaved caspase-1 relative fluorescence intensity. ^aP < 0.05. OC: Culture in vehicle adding equimolar sodium chloride to control for osmolality; DCA: Deoxycholic acid; ASP: Angelica sinensis polysaccharide; CA19-9: Carbohydrate antigen 19-9.