Angelica sinensis polysaccharide attenuates constipation-associated colorectal carcinogenesis by inhibiting the NLRP3/Caspase-1/IL-1β inflammasome axis

Figure 1 Angelica sinensis polysaccharide dose-dependently suppresses NOD-like receptor protein 3 expression in deoxycholic acid-stimulated NCM460 cells. A: Representative immunofluorescence images showing CK20 (green), NOD-like receptor protein 3 (red), DAPI (blue), and merged images in control, culture in vehicle adding equimolar sodium chloride to control for osmolality, deoxycholic acid, Angelica sinensis polysaccharide-L, Angelica sinensis polysaccharide-H, and nigericin groups; B: Quantification of NOD-like receptor protein 3 relative fluorescence intensity expressed as fold change vs control. ^aP < 0.05. OC: Culture in vehicle adding equimolar sodium chloride to control for osmolality; DCA: Deoxycholic acid; ASP: Angelica sinensis polysaccharide; CA19-9: Carbohydrate antigen 19-9.