Prognostic significance of Fusobacterium nucleatum in colorectal cancer using reconstructed survival data

Table 1 Characteristics and baseline data of included studies on Fusobacterium nucleatum in colorectal cancer

Study	Study interval	Country	Study design	Total cases, n	Positive cases, n	Biospecimen	Method	Group	Patients, n	Age, years	Male sex, %	Stage I, n	Stage II, n	Stage III, n	Stage IV, n
Ito et al[25], 2015	2001–2013	Japan, Asia	Cohort study	511	143	FFPE	qPCR	Negative	225	66.1 ± 11.9	57	23	63	112	27
								Low expression	143	69.1 ± 10.9	57	20	44	58	21
								High expression	143	66.5 ± 12.2	54	13	53	65	12
Kunzmann et al[26], 2019	2008–2018	Czech Republic, Europe	Cohort study	190	61	FF	qPCR	Low expression	129	65.6 ± 18.27	69	25	36	44	22 + (2 unknown stage)
								High expression	61	67 ± 17.6	67.2	9	23	14	14 + (1 unknown stage)
Oh et al[28], 2019	2005–2013	South Korea, Asia	Cohort study	593	204	FFPE	qPCR	Negative/low expression	389	56.6 ± 9	61.2	362		27
								High expression	204	66.3 ± 12	60.8	188		16
Chen et al[24], 2019	2017–2019	China, Asia	Cohort study	91	25	FFPE	qPCR	Negative/low expression	66	58.52 ± 10.42	57.6	-	36	30	-
								High expression	25	58.72 ± 12.64	68	-	15	10	-
Mima et al[27], 2016	Up to 2008	Japan, Asia	Cohort study	1069	67	FFPE	qPCR	Negative	935	69.2 ± 8.8	43	225	274	239	115
								Low	67	71.1 ± 9.0	39	9	23	25	5
								High	67	68.8 ± 8.3	34	7	28	14	13
Wei et al[31], 2016	2010–2012	China, Asia	Cohort study	180	90	FF	qPCR	Low expression	90	63.5 ± 10.2	63.3	53		37
								High expression	90	69.8 ± 12.5	56.7	42		48
Sun et al[30], 2016	2009–2010	China, Asia	Cohort study	152	59	FF	qPCR	Negative	34	66.5 ± 11.2	36.4	2	16	11	5
								Positive	118	69.2 ± 10.9	63.6	4	41	53	20
Yamaoka et al[32], 2018	1999–2008	Japan, Asia	Cohort study	100	50	FF	ddPCR	Low	50	66.7 ± 12.8	50	11	19	13	7
								High	50	67.0 ± 10.0	52	3	17	17	13
Yan et al[33], 2017	2007–2015	China, Asia	Cohort study	280	187	FFPE	qPCR	Low	93	65.3 ± 11.1	54.8	-	-
								High	187	68.4 ± 11.5	57.2	-	-
Sakamoto et al[22], 2021	2001–2018	Japan, Asia	Cohort study	181	8	FFPE	qPCR	Negative	173	63.5 ± 11.1	65.3	-	-
								Positive	8	58.5 ± 12.0	50	-	-
Phipps et al[29], 2025	1998–2007	United States, North America	Cohort study	859	86	FFPE	ddPCR	Negative	683	57 ± 12	48	212	375	80	16
								Low	90	58 ± 12	33.0	27	48	13	2
								High	86	58 ± 13	37	21	56	9	0
de Carvalho et al[23], 2019	2008–2015	Brazil, South America	Cohort study	152	18	FFPE	qPCR	Negative	117	60.6 ± 13.7	54.7	39	74	4	0
								Low	17	-	58.8	2	13	2	0
								High	18	-	38.9	2	15	0	0

Age is presented as mean ± SD. Expression categories reflect the definitions used in the original studies; when studies reported negative, low, and high categories, negative and low categories were combined as appropriate for the low/negative comparison group in the survival analysis. ddPCR: Digital droplet polymerase chain reaction; FF: Fresh-frozen tissue; FFPE: Formalin-fixed paraffin-embedded tissue; qPCR: Quantitative polymerase chain reaction; -: Data not reported or not available.