Enterocloster bolteae drives hyperglycemia-associated colorectal cancer cell proliferation via Rac/Rho-MSH6-Elongin C signaling pathway

Figure 3 Microbial community differences across groups. A: Relative abundance clustering heatmap. The clustering tree on the left represents species-level clustering. The values in the central heatmap correspond to Z-scores obtained by standardizing the relative abundance of each species. Linear discriminant analysis effect size analysis of species with intergroup differences comprises two parts; B: Linear discriminant analysis score bar chart. The horizontal axis represents group information (diabetes mellitus group, n = 8), diabetes mellitus-colorectal cancer group (n = 11), colorectal cancer group (n = 10), and control group (n = 11). The vertical axis represents species information; C: Evolutionary cladogram. Circles radiating from the inside outward represent taxonomic levels (e.g., genus or species). Each small circle at a given taxonomic level represents a specific taxon, and the diameter of the small circle is positively proportional to the relative abundance. Species with no significant differences are considered as a single group, and biomarkers of different species follow their respective group assignments. Orange nodes indicate microbial groups that play an important role in the orange module, while green nodes represent those important in the green module. The species corresponding to each letter in the cladogram are listed in the legend on the right; D: Krona chart visualizing species annotation results. Circles represent different taxonomic levels (phyla, families, genera, and species) from inside to outside. The size of each fan represents the relative proportion of the corresponding species. DM: Diabetes mellitus; CRC: Colorectal cancer; LDA: Linear discriminant analysis.