Transcription factor 3 enhances hepatocellular carcinoma metastasis by upregulating matrix metalloproteinase-11

Figure 3 Matrix metalloproteinase-11 is a direct transcriptional target of transcription factor 3. A: Differential gene expressions (DEGs) between Hep3B-transcription factor 3 (TCF3) and Hep3B-control through RT2 profiler polymerase chain reaction arrays test. The ordinate is a multiple of differences and threshold for DEGs was set as |log2 foldchange (FC)| > 3; B: Protein expression levels of matrix metalloproteinase-11 (MMP11) in treated Hep3B and HCCLM3 cells; C: Luciferase activities of reporter gene driven by the MMP11 promoter in hepatocellular carcinoma cells; D: The TCF3 binding motif logo obtain from JASPAR; E: Luciferase activities of the reporter gene driven by the serially truncated/mutated MMP11 promoter indicates TCF3 binding sites in Hep3B cells; F: Chip assay for binding of TCF3 to MMP11 promoter in Hep3B cells. Y-axis denotes enrichments with anti-TCF3 antibodies vs the immunoglobulin G control. ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. HCC: Hepatocellular carcinoma; IgG: Immunoglobulin G; MMP11: Matrix metalloproteinase-11; Sh: Short hairpin; TCF3: Transcription factor 3.