Rapamycin suppresses small bowel adenocarcinoma HUTU 80 cells proliferation by inhibiting hypoxia-inducible factor-1α mediated metabolic reprogramming

Figure 8 Rapamycin reduces the protein expression of phosphorylated-mammalian target of rapamycin, phosphorylated-eukaryotic translation initiation factor 4E-binding protein 1, phosphorylated-70 kDa ribosomal protein S6 kinase and hypoxia-inducible factor-1α in tumors in vivo. A: Protein levels were assayed after treatment of tumors with rapamycin, including mammalian target of rapamycin (mTOR), eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) and 70 kDa ribosomal protein S6 kinase (p70S6k), phosphorylated (p)-mTOR, p-4E-BP1 and p-p70S6k and hypoxia-inducible facto-1α; B: Quantification of protein expression using ImageJ. Data are presented as the mean ± SD (n = 3). ^aP < 0.05. ^bP < 0.01. RAPA: Rapamycin; mTOR: Mammalian target of rapamycin; p-mTOR: Phosphorylated-mammalian target of rapamycin; 4E-BP1: Eukaryotic translation initiation factor 4E-binding protein 1; p-4E-BP1: Phosphorylated-eukaryotic translation initiation factor 4E-binding protein 1; p70S6k: 70 kDa ribosomal protein S6 kinase; p-p70S6k: Phosphorylated-70 kDa ribosomal protein S6 kinase.