Rapamycin suppresses small bowel adenocarcinoma HUTU 80 cells proliferation by inhibiting hypoxia-inducible factor-1α mediated metabolic reprogramming

Figure 1 Effects of rapamycin on HUTU 80 cells proliferation. A: Methylthiazolyldiphenyl-tetrazolium bromide assay was used to assess the effect of rapamycin (RAPA) on the proliferation of HUTU 80 cells at 48 hours; B: Calculation of the IC₅₀ of RAPA on cells after 48 hours; C: Colony formation assay was used to assess the effect of RAPA on cell proliferation; D: Colony statistical chart. Data are presented as the mean ± SD (n = 3). ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. RAPA: Rapamycin; NS: No significance.