Methionyl-tRNA synthetase 1 participates in hepatocellular carcinoma and its regulated gene profile possesses potent prognostic ability

Figure 2 MARS1 promotes the proliferation, migration, invasion, and tumor formation of hepatocellular carcinoma cells. A: Protein expression levels of MARS1 in LM3 and Huh7 cells transiently transfected with siRNA-NC and siRNA-MARS1; B: Cell viability of LM3 and Huh7 cells measured by CCK-8 assay; C: Cell proliferation of LM3 and Huh7 cells examined by EdU assay; D: Cell cycle distribution of LM3 and Huh7 cells examined by cell cycle assays; E and F: Cell migration and invasion of LM3 and Huh7 cells assessed by Transwell migration and Matrigel invasion assays; G: Impact of MARS1 suppression in LM3 and Huh7 cells on subcutaneous tumor growth. The weight of tumors was measured following euthanasia of the mice (n = 5); H: Tumor volumes were recorded every 3 days (n = 5). Student’s t-test was used to compare differences between two groups, while one-way analysis of variance was applied for comparisons among more than two groups. ^aP < 0.05; ^bP < 0.01; and ^cP < 0.001.