Ginsenoside F4 inhibits colorectal cancer progression by boosting dendritic cell maturation and remodeling the tumor microenvironment

Figure 6 Effects of F4 on the immune cell infiltration in the tumor tissues of colon cancer CT26-bearing mice. A: Histological change of tumor tissues; B: Representative immunohistochemical images of Ki67 protein expressions in the tumor tissue; C: Representative immunofluorescent images indicated the infiltration of dendritic cells (DCs); D: Cluster of differentiation (CD) 8+ T cells in the tumor tissues. DCs were stained with Texas red-labeled CD11c antibody (red), CD8+ T cells were stained with Texas red-labeled CD8 antibody (red), and nuclei was stained with 4’,6-diamidino-2-phenylindole (blue); E: Quantification of DC or CD8+ T-cell infiltration was visualized by using three-dimensional Gaussian density analysis. All data were shown as mean ± SD (n = 5). ^aP < 0.05. P vs model group. Ctrl: Control; CAP: Capecitabine; L: Low; M: Middle; H: High; DC: Dendritic cells; CD: Cluster of differentiation; MFI: Mean fluorescence intensity.