Ginsenoside F4 inhibits colorectal cancer progression by boosting dendritic cell maturation and remodeling the tumor microenvironment

Figure 3 The regulation mechanism of F4 on the maturation of dendritic cells. A: The volcano plot of differently expressed genes in dendritic cells treated with F4 or saline. The pathways of the involved differently expressed genes were enriched by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis; B: The targets of F4 were predicted by SuperPred and SwissTargetPrediction web-tools. The binding energies among targets and F4 were detected by using AutoDock tool; C: The interaction between target and F4 was evaluated by using AutoDock tool; D: The schematic diagram of interaction between sphingosine-1-phosphate 1 (S1PR1) and F4; E: The interaction between S1PR1 and F4 was detected by surface plasmon resonance assay and verified by thermostability assay. FC: Fold change; TNF: Tumor necrosis factor; GO: Gene Ontology; LPS: Lipopolysaccharides; ERK: Extracellular regulated protein kinases; JAK: Janus kinase; STAT: Signal transducer and activator of transcription; PI3K: Phosphatidylinositol 3-kinase; AKT: Protein kinase B; S1PR1: Sphingosine-1-phosphate 1.