DEAD/H-box RNA helicase 10 promotes pancreatic cancer cell proliferation via ribonucleotide reductase M2

Figure 2 Knockdown of DEAD/H-box RNA helicase 10 inhibits the proliferation of pancreatic cancer cells. A: DEAD/H-box RNA helicase 10 (DDX10) mRNA expression by quantitative real-time PCR (qRT-PCR) in five pancreatic cancer cells. The value was represented with ∆Ct (DDX10-ACTB). ACTB was utilized as internal reference. All the reactions were run in triplicate. Data are represented as mean ± SD; B and C: Interference efficiency of two shRNAs for DDX10 was examined by qRT-PCR and Western blotting; D-F: Knockdown of DDX10 inhibits cell growth, as determined by counting kit-8 and colony formation assays; G: Gross observation of xenograft tumor size; H and I: Volume and weight of tumors derived from nude mouse models following DDX10 downregulation by shRNA were decreased in vivo. ^bP < 0.01, ^cP < 0.001; DDX10: DEAD/H-box RNA helicase 10; sh1: Sh1RNA-DEAD/H-box RNA helicase 10; sh2: Sh2RNA-DEAD/H-box RNA helicase 10.