Correlation between KAT6A and PD-L1 expression and role of KAT6A in colorectal cancer

Figure 6 Epigenetic editing of H3K23pr at PD-L1 promoter directly modulates immune evasion. A: CRISPR/dCas9-mediated H3K23pr editing efficiency evaluated by ChIP-qPCR analysis of H3K23pr enrichment at PD-L1 promoter in SW480 cells transfected with dCas9-p300 or dCas9-LSD1 and PD-L1-targeting sgRNA (5’-GCGCTCCAGCTCCGACCTGA-3’). Non-targeting sgRNA and empty vector served as controls; B: PD-L1 transcriptional regulation shown by quantitative PCR analysis of PD-L1 mRNA levels; C: Functional validation of immune evasion. Transwell invasion (left) and T cell-mediated apoptosis (right) assays; D: Rescue experiment in KAT6A-depleted cells. PD-L1 mRNA (left) and apoptosis (right) in siKAT6A cells co-transfected with dCas9-p300. Data represent the mean ± SEM (n = 3). ^cP < 0.001 vs non-targeting sgRNA (one-way ANOVA with Dunnett’s test). ^dP < 0.01.