Correlation between KAT6A and PD-L1 expression and role of KAT6A in colorectal cancer

Figure 2 KAT6A induces immune evasion in a colorectal cancer cell/T cell co-culture system. Colorectal cancer (CRC) cells treated with KAT6A siRNAs were co-cultured with unstimulated or anti-CD3/CD28-activated T cells (5 μg/mL each antibody, 72 hours of stimulation). A: T cell-mediated apoptosis of CRC cells was measured by flow cytometry; B: The expression of GZMB and perforin in T cells was analyzed by Western blot; C: The levels of PD-L1 in CRC cells were analyzed by Western blotting analysis.