BTNL9 exerts anti-cancer effects by inhibiting CDC20 to induce G2/M arrest in pancreatic cancer

Figure 4 Regulation of BTNL9 expression on downstream molecules. A: Reverse transcription quantitative PCR results of cell cycle related genes; B: The protein expression levels of genes with significant differences in reverse transcription quantitative PCR validation were detected by Western blot; C: Correlation analysis of CDC20 and BNTL9 expression in The Cancer Genome Atlas RNA sequencing data of pancreatic cancer; D: The expression of BTNL9 and CDC20 in pancreatic cancer cells with BTNL9 overexpression detected by immunofluorescence (green = green fluorescence protein; red= CDC20). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. GFP: Green fluorescence protein.