SAC3 domain containing 1 intervention in energy metabolism reprogramming assists in the progression of hepatocellular carcinoma

Figure 4 SAC3 domain containing 1 enhances the glycolysis and total adenosine triphosphate production in hepatocellular carcinoma cells. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. A: Gene set enrichment analysis indicated that SAC3 domain containing 1 (SAC3D1) promoted activation of the insulin signaling pathway; B: The Seahorse XFe96 assay was used to assess the impact of SAC3D1 on the total adenosine triphosphate production rate in hepatocellular carcinoma cells; C: The Seahorse XFe96 assay was utilized to evaluate the effect of SAC3D1 on the glycolytic rate in hepatocellular carcinoma cells; D: Real-time quantitative reverse transcription polymerase chain reaction was performed to examine the influence of SAC3D1 on the transcription levels of glycolysis-related genes; E: Western blotting was conducted to assess the effect of SAC3D1 on the expression of glycolysis-related proteins. SAC3D1: SAC3 domain containing 1; ROT/AA: Rotenone and antimycin A; NS: No significance; 2-DG: 2-deoxyglucose; GLUT1: Glucose transporter 1; HK2: Hexokinase 2; PKM2: Pyruvate kinase M2; LDHA: Lactate dehydrogenase A.