SAC3 domain containing 1 intervention in energy metabolism reprogramming assists in the progression of hepatocellular carcinoma

Figure 2 In vitro, SAC3 domain containing 1 promotes the proliferation of hepatocellular carcinoma cells. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. A: Real-time quantitative reverse transcription polymerase chain reaction was used to detect the transcriptional levels of SAC3 domain containing 1 (SAC3D1) in cell sublines with overexpressed and knocked down SAC3D1; B: Western blotting was employed to assess the protein levels of SAC3D1 in cell sublines with overexpressed and knocked down SAC3D1; C: Gene set enrichment analysis was conducted to explore the signaling pathways affected by the upregulation of SAC3D1 expression; D: Colony formation assays were conducted to validate the impact of SAC3D1 on the proliferative capacity of hepatocellular carcinoma cells; E: The 5-ethynyl-2′-deoxyuridine assay was performed to evaluate the impact of SAC3D1 overexpression on hepatocellular carcinoma cell proliferation, bar = 100 μm. SAC3D1: SAC3 domain containing 1; EdU: 5-ethynyl-2’-deoxyuridine.