Exploring the role of neutrophil extracellular traps in colorectal cancer: Insights from single-cell sequencing

Figure 3 Experimental procedures and association analysis for the study of intra-tumor heterogeneity in colorectal cancer. Firstly, a heterogeneous colorectal cancer (CRC) cell line is dissociated into single cell clones (SCCs), and their phylogenetic relationships are inferred. Subsequently, different SCC mixtures are inoculated into mice, and the mouse model is used to study the association between intra tumor heterogeneity and the immune response. Meanwhile, by integrating patient data, the relationships between intra tumor heterogeneity, patient survival, and immune checkpoint response are analyzed, aiming to comprehensively explore the mechanisms of intra tumor heterogeneity in CRC. CRC: Colorectal cancer; SCC: Single cell clone.