Exploring the role of neutrophil extracellular traps in colorectal cancer: Insights from single-cell sequencing

Figure 2 The workflow diagram of single cell RNA sequencing. Starting with cell suspension as the input, in library construction, 10 × barcoded gel beads with barcode and unique molecular identifier sequences are used, mixed with cells, enzymes, and oil to form single cell gel bead-in-emulsion and generate 10 × Barcoded complementary DNA. Then, the transcriptome is sequenced. Subsequently, in data analysis, bioinformatics pipelines like alignment, barcode processing, etc., are employed to generate a report. Finally, in data visualization, the analyzed data is presented graphically via computer software for easier interpretation of biological meaning. UMI: Unique molecular identifier; cDNA: Complementary DNA; GEMs: Gel bead-in-emulsion.