Nav1.6 drives colorectal cancer proliferation and invasion through MAPK signaling pathway

Figure 6 Knockdown of Nav1. 6 inhibits the RAF-MAPK signaling pathway. A: Volcano plot of differentially expressed genes (DEGs) before and after Nav1.6 knockdown; B: Heatmap of the top DEGs before and after Nav1.6 knockdown. Red indicates upregulated DEGs, and blue indicates downregulated DEGs; C: Expression of MAPK-related proteins MAP3K10, c-RAF, p-cRAF, ERK, p-ERK, and MEK1/2 before and after Nav1.6 knockdown in DLD-1 cells; D-G: Ratios of MAP3K10 to β-actin, p-ERK1/2 to total ERK1/2, p-cRAF to total c-RAF, and MEK1/2 to GAPDH in DLD-1 cells before and after Nav1.6 knockdown. ^aP < 0.05; ^cP < 0.001; ^dP < 0.0001; NS: Not significant.