Nav1.6 drives colorectal cancer proliferation and invasion through MAPK signaling pathway

Figure 5 Knockdown of Nav1. 6 inhibits the invasion and proliferation of colorectal cancer cells. A: Expression of Nav1.6 in three different human colorectal cancer cell lines; B and C: Wound healing assays of cell migration in CaCo2, HT-29, and DLD-1 cells. Images of wound closure are shown at the indicated time points (0, 12, and 24 hours) after scratching; D and E: Expression of Nav1.6 in DLD-1 cells before and after siRNA-mediated interference; F: Images of DLD-1 cells after transfection with Control siRNA or siNav1.6; G: Transwell invasion assay before and after Nav1.6 knockdown in DLD-1 cells; H: Statistical analysis of the Transwell invasion assay results; I: Cell viability of DLD1 cells at different time points after transfection with SiNav1.6. ^bP < 0.01; ^cP < 0.001.