Nav1.6 drives colorectal cancer proliferation and invasion through MAPK signaling pathway

Figure 2 Weighted correlation network analysis of the gene set associated with Nav1. 6 expression. A: Screening of SCN8A-correlated genes from tumors vs normal differentially expressed genes (DEGs) in the colon adenocarcinoma and rectal adenocarcinoma databases of The Cancer Genome Atlas; B: Volcano plot showing the upregulated genes (red) and downregulated genes in the 575 intersecting DEGs; C: Selection of soft-threshold power. Panel C shows the scale-free fit index (left) and average connectivity (right) vs soft-threshold power. A power of 12 was selected as the fit index curve flattens at high values (> 0.9); D: Cluster dendrogram and module assignment from weighted correlation network analysis. Genes were clustered based on dissimilarity measures (1-TOM). The branches represent modules of highly interconnected genes. The dynamic tree cut colors indicate modules identified by the Dynamic TreeCut method. Some modules were merged based on similarity, and the final merged modules were used for subsequent analysis; E: Heatmap representing the topological overlap matrix (TOM) of all genes in the analysis. The degree of overlap is indicated by color shading, with darker colors representing higher overlap and lighter colors indicating lower overlap. The gene dendrogram is shown on the left, and module assignments are shown on the top; F: Correlation of module eigengenes with clinical and pathological traits. Each row corresponds to a module eigengene, and the columns represent clinical traits. The values in the cells are presented as "Pearson r (P value)" and are color-coded based on the direction and magnitude of the correlation (red = positive correlation; blue = negative correlation). The 16 co-expressed transcript modules are shown along with their respective correlation parameters with clinical and pathological traits; G: Distribution of genes positively correlated with T stage in colorectal cancer (blue). READ: Rectal adenocarcinoma; COAD: Colon adenocarcinoma.