Flap endonuclease-1 promotes pancreatic cancer progression via AKT/mTOR signaling pathway

Figure 4 Flap endonuclease-1 regulates cell migration and expression of epithelial-mesenchymal transition-associated proteins in pancreatic cancer cells. A and B: Wound healing assays measuring cell migration dynamics in PATU8988T and PANC-1 cells following flap endonuclease-1 (FEN1) knockdown or overexpression. Migration rates were quantified at specified time points; C-F: Western blot analysis of epithelial-mesenchymal transition markers, including E-cadherin, N-cadherin, and Vimentin, in PATU8988T and PANC-1 cells with FEN1 knockdown or overexpression. Protein expression levels were normalized to internal controls. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.