Flap endonuclease-1 promotes pancreatic cancer progression via AKT/mTOR signaling pathway

Figure 3 Flap endonuclease-1 modulates proliferation, migration, and invasion in pancreatic cancer cells. A and B: Cell proliferation analysis using EdU incorporation assay in PATU8988T and PANC-1 cells following flap endonuclease-1 (FEN1) knockdown. Scale bar = 100 μm; C: Colony formation assays evaluating the proliferative capacity of PATU8988T and PANC-1 cells following FEN1 knockdown; D and E: Assessment of proliferative potential by EdU incorporation assay in PATU8988T and PANC-1 cells following FEN1 overexpression. Scale bar = 100 μm; F: Colony formation assays evaluating the proliferative capacity of PATU8988T and PANC-1 cells following FEN1 overexpression; G and H: Transwell invasion and migration assays assessing the metastatic potential of PATU8988T and PANC-1 cells following FEN1 knockdown or overexpression. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.