Flap endonuclease-1 promotes pancreatic cancer progression via AKT/mTOR signaling pathway

Figure 2 Flap endonuclease-1 knockdown and overexpression in pancreatic cancer cell lines. A and B: Validation of flap endonuclease-1 (FEN1) knockdown efficiency in PATU8988T and PANC-1 cells using two independent shRNA constructs, as assessed by reverse transcription-quantitative PCR (RT-qPCR) and western blot analyses for mRNA and protein expression, respectively; C and D: Verification of FEN1 overexpression in PATU8988T and PANC-1 cells, evaluated by RT-qPCR for mRNA levels and western blot analysis for protein expression. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.