Tumor-derived exosomal miR-425-5p and miR-135b-3p enhance colorectal cancer progression through immune suppression and vascular permeability promotion

Figure 1 Exosomal miR-425-5p and miR-135b-3p are upregulated in colorectal cancer patients and cell lines. A: Heatmap; B: Volcano plot of differentially expressed tissue exosomal microRNAs (miRNAs) between tumors tissues and normal adjacent tissues of colorectal cancer patients; C: Real-time quantitative polymerase chain reaction validation of miR-425-5p and miR-135b-3p from exosomes of colorectal cancer cell lines (SW480, HT-29, SW620, HCT116) and normal cells (FHC); D: Kyoto Encyclopedia of Genes and Genomes enrichment analyses of miR-425-5p and miR-135b-3p; E: Transmission electron microscopy images; F: Nanoparticle tracking analysis of exosomes isolated from HCT116 cells; G: Western blot analysis of exosomes isolated from HCT116 cells. ^aP < 0.05 vs FHC-exo (n = 3 per group). NC-exo: Negative control-exosome; TSG101: Tumor susceptibility gene 101 protein.