Tumor-derived exosomal miR-425-5p and miR-135b-3p enhance colorectal cancer progression through immune suppression and vascular permeability promotion

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Jun 15, 2025 (publication date) through Jun 19, 2025

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World Journal of Gastrointestinal Oncology

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1948-5204

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastrointest Oncol. Jun 15, 2025; 17(6): 106161
Published online Jun 15, 2025. doi: 10.4251/wjgo.v17.i6.106161

Table 1 Primer sequence for real-time quantitative polymerase chain reaction

Gene	Primer sequence (5’-3’)
U6	Forward: CTCGCTTCGGCAGCACA
U6	Reverse: AACGCTTCACGAATTTGCGT
MiR-425-5p	Forward: AAUGACACGAUCACUCCCGUUGA
MiR-425-5p	Reverse: AGTGCAGGGTCCGAGGTATT
MiR-135b-3p	Forward: AUGUAGGGCUAAAAGCCAUGGG
MiR-135b-3p	Reverse: AGTGCAGGGTCCGAGGTATT
GAPDH	Forward: AATGACCCCTTCATTGAC
GAPDH	Reverse: TCCACGACGTACTCAGCGC
IL-1β	Forward: AACCTCTTCGAGGCACAAGG
IL-1β	Reverse: AGATTCGTAGCTGGATGCCG
IL-6	Forward: CCTTCGGTCCAGTTGCCTTCT
IL-6	Reverse: TCTGAGGTGCCCATGCTACA
TNF-α	Forward: CTGAGACTCTCCTGTGCAGC
TNF-α	Reverse: ACTCCCTGAAGAGACGGTGA
IL-4	Forward: CTTTGCTGCCTCCAAGAACA
IL-4	Reverse: GTTCCTGTCGAGCCGTTTCA

IL-1β: Interleukin 1 beta; IL-6: Interleukin 6; TNF-α: Tumor necrotic factor alpha; IL-4: Interleukin 4.

Citation: Feng CZ, Zhong SQ, Ye SW, Zheng Z, Sun H, Zhou SH. Tumor-derived exosomal miR-425-5p and miR-135b-3p enhance colorectal cancer progression through immune suppression and vascular permeability promotion. World J Gastrointest Oncol 2025; 17(6): 106161
URL: https://www.wjgnet.com/1948-5204/full/v17/i6/106161.htm
DOI: https://dx.doi.org/10.4251/wjgo.v17.i6.106161