Regulator of chromosome condensation 1 promotes hepatocellular carcinoma proliferation via cell-division-cycle-associated-8 dependent phosphoinositide 3-kinase/protein kinase B signaling

Figure 6 Regulator of chromosome condensation 1 regulates phosphoinositide 3-kinase/protein kinase B/cyclin-dependent kinase inhibitor 1a pathway through cell division cycle-associated 8 in hepatocellular carcinoma. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP< 0.0001. A: Immunoblotting analysis of phosphatidylinositol - 4,5 - bisphosphate 3 - kinase catalytic subunit alpha (PIK3CA), protein kinase B (Akt), p-Akt and cyclin-dependent kinase inhibitor 1a (p21) in vector and regulator of chromosome condensation 1-overexpressing Huh-7 cells. Actin was used as the loading control; B: Quantification of PIK3CA, Akt, p-Akt and p21 expression normalized to actin; C: Immunoblotting analysis of PIK3CA, Akt, p-Akt and p21 in small interfering RNA negative control and regulator of chromosome condensation 1-knockdown Huh-7 cells. Actin was used as the loading control; D: Quantification of PIK3CA, Akt, p-Akt and p21 expression normalized to actin; E: Immunoblotting analysis of PIK3CA, Akt, p-Akt and p21 in different treatment Huh-7 cells. Actin was used as the loading control; F: The quantification of PIK3CA, Akt, p-Akt and p21 expression normalized to actin. RCC1: Regulator of chromosome condensation 1; siNC: Small interfering RNA negative control; CDCA: Cell division cycle-associated; PIK3CA: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; Akt: Protein kinase B.