Regulator of chromosome condensation 1 promotes hepatocellular carcinoma proliferation via cell-division-cycle-associated-8 dependent phosphoinositide 3-kinase/protein kinase B signaling

Figure 5 Silencing cell division cycle-associated 8 attenuates the proliferative enhancement induced by regulator of chromosome condensation 1 overexpression in hepatocellular carcinoma cells. ^dP < 0.0001. A: Spearman correlation analysis of regulator of chromosome condensation 1 with cell division cycle-associated (CDCA) 8, minichromosome maintenance complex component 4, cyclin B1 and CDCA5 expression; B: Immunoblotting analysis of the interaction between regulator of chromosome condensation 1 and CDCA8 in Huh-7 cell line; C and D: Cell counting kit-8 assay and 5-ethynyl-2’-deoxyuridine incorporation assay detected proliferation in Huh-7 cells under different treatments; E: Statistical results of ethynyl-2’-deoxyuridine-positive cells percentage from Figure 5D; F and G: Cell cycle analysis and the statistical results in different treatment Huh-7 cells. RCC1: Regulator of chromosome condensation 1; CDCA: Cell division cycle-associated; IP: Immunoprecipitation; siNC: Small interfering RNA negative control; IgG: Immunoglobulin G.