Regulator of chromosome condensation 1 promotes hepatocellular carcinoma proliferation via cell-division-cycle-associated-8 dependent phosphoinositide 3-kinase/protein kinase B signaling

Figure 4 Regulator of chromosome condensation 1 modulates the expression of cell cycle-associated genes in hepatocellular carcinoma. ^aP < 0.05, ^dP < 0.0001. A: Heatmap displaying the genes correlated with regulator of chromosome condensation 1 (RCC1) in hepatocellular carcinoma samples from The Cancer Genome Atlas database; B: Enrichment analysis of genes with correlation coefficients exceeding 05; C-E: The mRNA and protein expression analysis of cell division cycle-associated (CDCA) 8, minichromosome maintenance complex component, cyclin B1 and CDCA5 in vector and RCC1-overexpressing Huh-7 cells; F-H: Quantitative reverse transcription polymerase chain reaction and immunoblotting analysis of CDCA8, minichromosome maintenance complex component 4, cyclin B1 and CDCA5 in small interfering RNA negative control and RCC1-knockdown Huh-7 cells. RCC1: Regulator of chromosome condensation 1; CDCA: Cell division cycle-associated; MCM4: Minichromosome maintenance complex component 4; CCNB1: Cyclin B1; siNC: Small interfering RNA negative control.