Regulator of chromosome condensation 1 promotes hepatocellular carcinoma proliferation via cell-division-cycle-associated-8 dependent phosphoinositide 3-kinase/protein kinase B signaling

Figure 3 Knockdown of regulator of chromosome condensation 1 inhibits the proliferation and motility of hepatocellular carcinoma cells. ^cP < 0.001, ^dP < 0.0001. A: Immunoblotting analysis of regulator of chromosome condensation 1 (RCC1) in small interfering RNA negative control (siNC) and RCC1-knockdown Huh-7 cells. Actin was used as the loading control; B: Quantification of RCC1 expression normalized to actin according to Figure 3A; C and D: Cell counting kit-8 assay and 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay detected proliferation in siNC and RCC1-knockdown Huh-7 cells; E: Statistical results of 5-ethynyl-2’-deoxyuridine-positive cell percentage from Figure 3D; F and G: Cell cycle analysis and the statistical results between Huh-7-siNC and Huh-7-siRCC1; H: Wound healing assay detected the migration in siNC and RCC1-knockdown Huh-7 cells; I: Statistical results of wound closure percentage from Figure 3H; J: Transwell invasion assay detected the invasion in Huh-7-siNC and Huh-7-siRCC1; K: Statistical results of cell numbers from Figure 3J. siNC: Small interfering RNA negative control; RCC1: Regulator of chromosome condensation 1.