Regulator of chromosome condensation 1 promotes hepatocellular carcinoma proliferation via cell-division-cycle-associated-8 dependent phosphoinositide 3-kinase/protein kinase B signaling

Figure 2 Regulator of chromosome condensation 1 overexpression promotes the cell growth and motility of hepatocellular carcinoma cells. ^cP < 0.001, ^dP < 0.0001. A: Immunoblotting analysis of regulator of chromosome condensation 1 (RCC1) in vector and RCC1-overexpressing Huh-7 cells, with actin as the loading control; B: Quantification of RCC1 expression normalized to actin according to Figure 2A; C and D: Cell counting kit-8 assay and 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay detected the proliferation in vector and RCC1-overexpressing Huh-7 cells; E: Statistical results of 5-ethynyl-2’-deoxyuridine-positive cell percentage from Figure 2D; F and G: Cell cycle analysis and the statistical results between Huh-7-vector and Huh-7-RCC1 cells; H: Wound healing assay detected the migration in vector and RCC1-overexpressing Huh-7 cells; I: Statistical results of wound closure percentage from Figure 2H; J: Transwell invasion assay detected the invasion in Huh-7-vector and Huh-7-RCC1; K: Statistical results of cell numbers from Figure 2J. RCC1: Regulator of chromosome condensation 1.